Phenolics extraction and use

ABSTRACT

Methods for concentrating phenolics in a solution and compositions related to the concentrated phenolics are provided In particular, methods and compositions are described relating to concentrated phenolics obtained from cranberry feedstock extracts combined with fumaric acid as beverage additives Additionally, methods for obtaining phenolics from feedstocks using resin absorption and elution are described.

CLAIM OF PRIORITY

This application claims priority to U.S. Provisional Application Ser.No. 61/170,090, filed on Apr. 16, 2009, the content of which is herebyincorporated by reference in its entirety.

TECHNICAL FIELD

The present disclosure provides, inter alia, compositions and processesrelated to the concentration of phenolics.

BACKGROUND

Many phenolics, e.g., plant derived phenolics (e.g., phenolics in fruitsand vegetables), can be useful in food stuffs and/or as health products(e.g., dietary supplements), due to their well documented associationwith human health. Certain foods, for example, fruits such ascranberries, provide a rich source of phenolics. However, currentmethods for the recovery of plant derived phenolics are inefficient oryield undesirable mixtures of phenolics. Improved methods for theselective recovery and/or concentration of phenolics are desired. Suchmethods may allow novel opportunities in the field of health productdevelopment.

SUMMARY

Compositions and processes are provided for extracting, obtaining and/orconcentrating (e.g., enriching) phenolics.

In sonic aspects, the present disclosure provides methods of extractingphenolics. These methods can include steps of obtaining a liquidfeedstock. Useful liquid feedstocks can include any phenolics containingfeedstock. In some embodiments, the liquid feedstock includes a juiceobtained from one or more phenolics containing fruits, vegetables,legumes and the like. For example, liquid feedstock can include juiceobtained from cranberries (e.g., cranberry juice). Cranberry juice canbe obtained by any method that allows juice to be obtained fromcranberries. In some instances, cranberry juice can be obtained bycrushing cranberries and purifying the resulting cranberry juice.Methods suitable for use in such application are known in the art andinclude, but are not limited to, e.g., counter current extraction.Liquid feedstock can then be contacted with a material that retains,captures, or binds phenolics, and that does not substantially retain,capture, or bind sugars and organic acids. The duration of time andconditions under which the feedstock is contacted with such a material(e.g., a resin) can be modified such that at least a portion of thephenolics present in the liquid feedstock are retained, captured, orbound, without retaining, capturing, or binding at least sugars andorganic acids. In some aspects, the liquid feedstock is contacted with aresin. Suitable resins include, but are not limited to, for example,resins with one or more of the following physical properties: a surfacearea of greater than or equal to about 300 m²/g (e.g., greater than 380m²/g or equal to about 700 m2/g), aliphatic ester resins, a moistureholding capacity of about 61% to about 69%, a porosity of greater thanabout 0.5 ml/ml. In some aspects, the resin can include Amberlite™XAD-7HP resin. In some aspects the resin can include Amberlite™ FPX-66.The material contacted with the liquid feedstock (e.g., the resin) canthen be washed using a solution that does not substantially reduce theamount of phenolics retained, captured, or bound therein. Useful washsolutions can include a solvent diluted in water to a concentration thatdoes not substantially reduce the amount of phenolics retained,captured, or bound to the material (e.g., the resin). Exemplary solventsinclude, for example, ethanol at a concentration of about 5% by volume.The portion of phenolics retained, captured, or bound to the material(e.g., the resin) can then be obtained using a solution comprising asolvent, wherein the elution solution substantially decreases the amountof phenolics bound to the resin. Suitable elution solutions can include,but are not limited to, for example, about 95% ethanol by volume orabout 90% acetone by volume. In some embodiments, the resultingphenolics can be obtained. Additional steps to remove any solventpresent in the phenolics solution can optionally be performed, and/orthe solution can be concentrated.

The phenolics containing solution resulting from the above describedprocess is referred to herein as an extract. Such extracts can includephenolics at a second concentration, wherein the second concentration isgreater than the first concentration and wherein the first concentrationis the concentration of the phenolics present in the liquid feedstock.Further, the extract can include at least anthocyanins andproanthocyanidins (PACs) and one or more of the following: a ratio ofanthocyanins to PACs of about 1:5; a PACs oligomeric profile that issubstantially the same as the PACs oligomeric profile in cranberries; aratio of PACs to total phenolics that is substantially the same as theratio of PACs to total phenolics in cranberries; a ratio of PACs toanthocyanins that is not the same as the ratio of PACs to anthocyaninsin cranberries; phenolics with an average molecular weight of less than14,000 Daltons; less than about 5% organic acids; and/or less than about5% sugars. Such extracts, if liquid, can be dried to thereby provide adry extract.

In some aspects, the present disclosure provides methods of making abeverage suitable for ingestion by a subject (e.g., a human or non-humansubject). Such methods can include obtaining phenolics using the processdescribed above, and adding the resulting extract to a liquid suitablefor ingestion by a human and/or non-human animal. Exemplary beveragescan include, but are not limited to beverages that contain fruit juiceor juices.

In some aspects, the present disclosure provides phenolics containingextracts. Such extracts can include at least anthocyanins andproanthocyanidins (PACs) and at least one of the followingcharacteristics or properties: a ratio of anthocyanins to PACs of about1:5; a PACs oligomeric profile that is substantially the same as thePACs oligomeric profile in cranberry juice or cranberries; a ratio ofPACs to total phenolics that is substantially the same as the ratio ofPACs to total phenolics in cranberry juice or cranberries; a ratio ofPACs to anthocyanins that is not the same as the ratio of PACs toanthocyanins in cranberry juice or cranberries; phenolics with anaverage molecular weight of less than 14,000 Daltons; less than about 5%organic acids; and/or less than about 5% sugars. These extracts can beliquid, dry, or partially dry (e.g., dehydrated, lyophilized, orpowdered), or gel extract.

In some aspects, the present disclosure provides compositions thatinclude at least anthocyanins and proanthocyanidins (PACs) and at leastone of the following characteristics or properties: a ratio ofanthocyanins to PACs of about 1:5; a PACs oligomeric profile that issubstantially the same as the PACs oligomeric profile in cranberry juiceor cranberries; a ratio of PACs to total phenolics that is substantiallythe same as the ratio of PACs to total phenolics in cranberry juice orcranberries; a ratio of PACs to anthocyanins that is not the same as theratio of PACs to anthocyanins in cranberry juice or cranberries;phenolics with an average molecular weight of less than 14,000 Daltons;less than about 5% organic acids; and/or less than about 5% sugars.These extracts can be liquid, dry, or partially dry (e.g., dehydrated,lyophilized, or powdered), or gel extract.

In some aspects, the present disclosure provides compositions thatinclude fumaric acid (e.g., isolated or purified fumaric acid) andphenolics (e.g., isolated, purified, or enriched phenolics (e.g.,PACs)). In some embodiments, the ratio of fumaric acid to phenolics(e.g., proanthocyanidins (PACs) within such compositions is betweenabout 4000:1 to about 100:1. For example, the ratio can be about 135:1,or about 238:1. In some embodiments, these compositions can be presentin a beverage. Such beverages can have a pH of between about pH 2.0 toabout pH 3.49. In some embodiments, the pH of such beverages can beequal to or greater than pH 3.49. For example, the pH can be about pH2.0 to about pH 4.1 (e.g., pH 3.7 or pH 4.1). In some embodiments, thebeverage can be a beverage that includes apple juice or the beverage canbe apple juice. In some aspects, compositions comprising isolatedfumaric acid and isolated phenolics can include a ratio of fumaric acidto phenolics (e.g., PACs) of between about, e.g., 10:1-50:1, or about14:1. Such compositions can also be presented in a beverage. In someinstances, such beverages can have a pH of greater than or equal toabout pH 3.5, and/or the beverage can contain orange juice. In someembodiments, the phenolics present in these compositions can include,e.g., at least anthocyanins and PACs, and one of the following of thefollowing: a ratio of anthocyanins to PACs of about 1:5; a PACsoligomeric profile that is substantially the same as the PACs oligomericprofile in cranberry juice or cranberries; a ratio of PACs to totalphenolics that is substantially the same as the ratio of PACs to totalphenolics in cranberry juice or cranberries; a ratio of PACs toanthocyanins that is not the same as the ratio of PACs to anthocyaninsin cranberry juice or cranberries; phenolics with an average molecularweight of less than 14,000 Daltons; less than about 5% organic acids;and/or less than about 5% sugars. Alternatively, or in addition, thephenolics can be obtained using the methods disclosed herein. In someembodiments, compositions that include fumaric acid (e.g., isolated orpurified fumaric acid) and phenolics (e.g., isolated, purified, orenriched phenolics (e.g., PACs)) can be powdered or liquid. In someembodiments, compositions that include fumaric acid (e.g., isolated orpurified fumaric acid) and phenolics (e.g., isolated, purified, orenriched phenolics (e.g., PACs)) can be in a container. In someembodiments, the composition comprises isolated fumaric acid andisolated phenolics, e.g. within a suitable container.

In some aspects, the disclosure provides beverages (e.g., beveragessuitable for ingestion by a human or non-human animal (e.g., fruit juicebeverages) that includes phenolics containing at least PACs, and fumaricacid. In such aspects the concentration of PACs can be between about4.2×10⁻⁴ mg/mL and 8.29×10⁻³ mg/mL and the concentration of fumaric acidcan be between about 0.01% (weight/volume (w/v)) and 0.15% (w/v). Insome embodiments the concentration of PACs can be about 4.2×10⁻³ mg/mLand the concentration of fumaric acid can be about 0.1% (w/v). In someinstances, the pH of beverages containing such compositions can bebetween about pH 2.0 to about pH 3.49, or the beverage can be applejuice or a beverage comprising apple juice. In some embodiments, thephenolics in such beverages can include at least anthocyanins andproanthocyanidins (PACs) and at least one of the following: a ratio ofanthocyanins to PACs of about 1:5; a PACs oligomeric profile that issubstantially the same as the PACs oligomeric profile in cranberry juiceor cranberries; a ratio of PACs to total phenolics that is substantiallythe same as the ratio of PACs to total phenolics in cranberry juice orcranberries; a ratio of PACs to anthocyanins that is not the same as theratio of PACs to anthocyanins in cranberry juice or cranberries;phenolics with an average molecular weight of less than 14,000 Daltons;less than about 5% organic acids; and/or less than about 5% sugars, orthe phenolics can be obtained using the methods disclosed herein.

In some aspects, such beverages can include a concentration of PACs ofbetween about 4.2×10⁻⁴ mg/mL and 0.1 mg/mL and a concentration offumaric acid of between about 0.01% (weight/volume (w/v)) and 0.15%(w/v). For example, in some instances the concentration of PACs can beabout 5×10⁻² mg/mL and the concentration of fumaric acid can be about0.07% (w/v). In some instances, the pH of beverages containing suchcompositions can be greater than or equal to about pH 3.5 and/or thejuice can be a beverage comprising orange juice.

In some aspects, the present disclosure provides methods of makingcompositions for reducing bacterial contamination (e.g.,Alicyclobacillus (ACB) contamination) of a beverage. Such method caninclude obtaining isolated fumaric acid and isolated phenolicscomprising PACs; and combining the isolated phenolics and fumaric acidto yield a ratio of fumaric acid to PACs of between about 4000:1 toabout 100:1 when the composition is added to the beverage. In someembodiments, the ratio can be about 135:1 or about 238:1.

In some aspects, the present disclosure provides methods of makingcompositions for reducing bacterial contamination (e.g.,Alicyclobacillus (ACB) contamination) of a beverage. Such methods caninclude obtaining isolated fumaric acid and isolated phenolicscomprising PACs; and combining the isolated phenolics and fumaric acidto yield a ratio of fumaric acid to PACs of between about 10:1 to about50:1. In some embodiments, the ratio can be about 14:1.

In some aspects, the present disclosure encompasses methods of making abeverages that include obtaining a beverage; and adding to the beveragephenolics comprising PACs and fumaric acid, wherein the finalconcentration of exogenously added PACs is between about 4.2×10⁻⁴ mg/mLand 8.29×10⁻³ mg/mL and the concentration of fumaric acid is betweenabout 0.01% (weight/volume (w/v)) and 0.15% (w/v). In some embodiments,the concentration of PACs can be about 4.2×10⁻³ mg/mL and theconcentration of fumaric acid is about 0.1% (w/v), and/or the pH of thebeverage can be between about pH 2.0 to about pH 3.49. In someembodiments, the beverage can be apple juice or a beverage comprisingapple juice. In such embodiments, the concentration of exogenously addedPACs can be about 4.2×10³ mg/mL and the concentration of fumaric acidcan be 0.1% (w/v). In some embodiments, the phenolics included in suchbeverages can include at least anthocyanins and proanthocyanidins (PACs)and at least one of the following: a ratio of anthocyanins to PACs ofabout 1:5; a PACs oligomeric profile that is substantially the same asthe PACs oligomeric profile in cranberry juice or cranberries; a ratioof PACs to total phenolics that is substantially the same as the ratioof PACs to total phenolics in cranberry juice or cranberries; a ratio ofPACs to anthocyanins that is not the same as the ratio of PACs toanthocyanins in cranberry juice or cranberries; phenolics with anaverage molecular weight of less than 14,000 Daltons; less than about 5%organic acids; and/or less than about 5% sugars. In some embodiments,these methods can include adding to the beverage phenolics comprisingPACs and fumaric acid, wherein the final concentration in the beverageof exogenously added PACs is between about 4.2×10⁻⁴ mg/mL and 0.1 mg/mLand the concentration of fumaric acid is between about 0.01%(weight/volume (w/v)) and 0.15% (w/v). In some embodiments, theconcentration of PACs can be about 5×10⁻² mg/mL and the concentration offumaric acid can be about 0.07% (w/v), and/or the beverage can have a pHof greater than or equal to about pH 3.5, and/or the beverage can beorange juice or a beverage comprising orange juice. In some embodiments,the beverage is orange juice and the concentration of exogenously addedPACs is about 5×10⁻² mg/mL and the concentration of fumaric acid is0.07% (w/v).

Unless otherwise defined, all technical and scientific terms used hereinhave the same meaning as commonly understood by one of ordinary skill inthe art to which this invention belongs. Methods and materials aredescribed herein for use in the present invention; other suitablemethods and materials known in the art can also be used. The materials,methods, and examples are illustrative only and not intended to belimiting. All publications, patent applications, patents, sequences,database entries, and other references mentioned herein are herebyincorporated by reference in their entirety. In case of conflict, thepresent specification, including definitions, will control.

Other features and advantages of the invention will be apparent from thefollowing description and figures, and from the claims.

DESCRIPTION OF DRAWINGS

FIG. 1 is a flow diagram providing one embodiment of the processdisclosed herein. A through K correspond to the Sample IDs shown inTable 7.

FIG. 2 is a line graph showing the growth of Alicyclobacillus species inthe presence of concentrations of extract and fumaric acid.

FIGS. 3A-3B are bar graphs showing the log number of AlicyclobacillusCFU in apple juice treated with extract (CE, mg PAC/8 oz) and/or fumaricacid (%). Mean+sem, n=3. (A) Significant differences are indicated bydifferent letters. (B) Log reduction in the number of AlicyclobacillusCFU.

FIGS. 4A-4B are bar graphs showing the log number of AlicyclobacillusCFU in orange juice treated with extract (CE, mg PAC/8 oz) and/orfumaric acid (%). Mean+sem, n=3. (A) Significant differences areindicated by different letters. (B) Log reduction in the number ofAlicyclobacillus CFU.

DETAILED DESCRIPTION

The present disclosure is based, at least in part, on the finding thatphenolics can be extracted, obtained, and/or concentrated from afeedstock containing phenolics using the process disclosed herein.Furthermore, the present disclosure provides that these extractedphenolics can be used in the development of health products and foodpreservatives. Accordingly, the present disclosure provides, inter alia,processes that can be used to extract, obtain, and/or concentrate (e.g.,enrich) phenolics (e.g., naturally occurring phenolics, e.g., plantphenolics) from feedstocks containing phenolics, and compositions (e.g.,extracts) containing phenolics obtained using these processes (e.g.,phenolics enriched extracts). Such compositions—which are termed hereinas “phenolics,” “enriched extracts” or simply “extracts”—can include,for example, at least anthocyanins and proanthocyanidins (PACs).

Processes

Referring to FIG. 1, a flow diagram is provided illustrating oneexemplary embodiment of a process for extracting phenolics from aphenolics containing feedstock (e.g., cranberries or a solution obtainedfrom cranberries). The process can use a feedstock that containsphenolics. This feedstock can be a solid or liquid. Solid feedstocks canbe liquefied or solubilized and optionally filtered to generate a liquidfeedstock prior to use in the disclosed process.

The process can begin with a phenolics containing liquid feedstock(e.g., cranberry juice obtained by countercurrent extraction (CCE), asdescribed in U.S. Pat. Nos. 5,320,861 and 5,419,251). Liquid feedstockscan contain known amounts of solids per volume (e.g., about 5 pounds ofsolids per gallon or about 50 Brix). If required (e.g., to decrease orincrease the concentration of the solids in the liquid), the liquidfeedstock can be diluted (e.g., using water or reverse osmosis (RO)water) in “mix tank” 10 to yield a lower concentration of solids pervolume (e.g., about 2.3 pounds of solids per gallon or about 25 Brix),or concentrated to yield a higher concentration of solids per volume(e.g., about 8.6 pounds of solids per gallon or about 75 Brix). Theconcentration of solids in the liquid feedstock can be modified to yielda viscosity, e.g., for allowing optimal passage or flow of the materialthrough columns 20 and 30 (e.g., the concentration of solids can beabout 0.01, 0.05, 0.1, 0.5, 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5, 5.5, 6,6.5, 7, 7.5, or about 8.6 pounds of solids per gallon). The liquidfeedstock can be held in mix tank 10 prior to being fed to columns 20and 30.

Prior to being contacted with the liquid feedstock, resin in columns 20and 30 can be contacted with a volume of liquid (e.g., water or ReverseOsmosis (RO) water) sufficient to remove or dilute media (e.g., ethanol)used to store the resin. The liquid feedstock can then be fed from mixtank 10 to resin columns 20 and 30, wherein the resin is contacted bythe liquid feedstock for a time and under conditions sufficient forphenolics (e.g., a portion of phenolics) present in the feedstock tobind to the resin (e.g. are captured or retained by the resin). Liquidexiting columns 20 and 30 is referred to as feedstock flow through. Aninitial volume of feedstock flow through can be discarded as waste orredirected for further processing. The remaining volume of feedstockflow through can be collected as reduced-phenolics containing permeate.

A volume of wash solution can then be fed from wash tank 50 to resincolumns 20 and 30 to remove residual feedstock. All wash solution flowthrough can be collected as permeate.

A volume of elution solution containing a solvent can subsequently befed from elution tank 60 to resin columns 20 and 30 to remove boundphenolics (e.g., a substantial portion of bound phenolics) from theresin. The entire volume, or a portion thereof, of elution solution flowthrough can be collected in holding vessel 70.

The above steps represent a single cycle of the exemplary process. Thiscycle can be repeated any number of times (e.g., 2, 3, 4, 5, 6, 7, 8, 9,10, or more, times), as required to obtain a target volume of elutionsolution, in a single run. Repeat cycles can be batch type,semi-continuous, or continuous. Prior to additional cycles, the columnscan be flushed. Alternatively, if further cycles are not required, resinin columns 20 and 30 can be submerged in ethanol for storage.

When required, e.g., once a sufficient volume of elution solution flowthrough has been collected in holding tank 70, the elution solution flowthrough can be fed to evaporator feed tank 80 and then cycled through toevaporator 90 and flash pot 100 (e.g., once or multiple times or asrequired to reduce the solvent content of the elution solution flowthrough to less than 90 parts per million using, e.g., evaporator 130),where much of the solvent is recovered by evaporation to yield a volumeof solution containing a reduced amount of solvent and water andconcentrated phenolics. The reduced solvent solution (i.e., the extract,i.e., the liquid extract containing, e.g., water and extractedphenolics) can be fed to tank 110 and/or tote 120.

In some instances, the liquid extract can optionally be dried, e.g.,spray dried.

The above described steps constitute a single run of a single process.

The foregoing is a description of one embodiment of the process. Thoseskilled in the art will be able to modify the process and willappreciate that any number of variations are possible and within thepresent disclosure.

As noted above, the process disclosed herein can include one or morecycles encompassed by a single run. Runs can be repeated as required.

Feedstock that can be used in the foregoing process can include anynaturally occurring and/or synthetic materials (e.g., solutions andliquids) containing phenolics (e.g., containing levels (e.g., naturallyoccurring levels) of at least anthocyanins and/or proanthocyanidins(e.g., type A proanthocyanidins)). Phenolics can include, for example,the art recognized class of compounds, which may also be known asphenols, and all compounds known in the art to be encompassed by thisclass of compounds. The phenolics class encompasses a diverse range ofnaturally occurring and synthetic compounds. The simplest of thephenolics is phenol, which contains a single hydroxyl group directlybonded to an aromatic group. Phenolics also include the polyphenols,which contain more than one phenol unit per molecule. The most commonlyoccurring polyphenols are classified as flavonoids. All flavonoidscontain a nucleus consisting of two phenolic rings and an oxygenatedheterocycle. Flavonoids are further categorized, based upon theiroxidation state. Exemplary classes of flavonoids include flavonols,flavanols, catechins, flavanones, anthocyanidins, and isoflavonoids.Proanthocyanidins (PACs), or condensed tannins, are a form of flavanolthat are composed of polymer chains of catechins. (see e.g., CheynierV., Am. J. Clin. Nutr, 81:223 S-229S, 2005). PACs are reported to beformed through reactions of anthocyanins with compounds containing apolarizable double bond. Accordingly, PACs differ in the nature of theirconstitutive units, sequence, the positions of interflavanic linkages,chain length, and the presence of substituents (e.g., galloyl orglucosyl groups). PACs, including higher-molecular weight PACs, aregenerally soluble in aqueous media or hydroalcoholic media. PAC proteinaffinity and astringency correlates (e.g., increases) with the degree ofPAC polymerization and galloylation. Specifically, higher-molecularweight PACs are more astringent than oligomeric PACs (Vidal et al., J.Sci. Food Agric., 83:564-573, 2003). Furthermore, PACs with a low degreeof polymerization, e.g., a degree of polymerization of 2 to 4 (dimer totetramer), i.e., oligomeric PACs, are highly bioactive. Exemplary listsof compounds encompassed by the class are publicly available and can befound, for example, on the World Wide Web (see, for example, World WideWeb address en.wikipedia.org/wiki/Category:Phenols (accessed on Nov. 27,2009, and last modified on 23 Nov. 2009 at 14:07), in text books, and inpublished periodicals. The term phenolics includes those compoundsencompassed by the phenols, polyphenols, flavonols, flavanols,catechins, flavanones, anthocyanidins, and isoflavonoids, andproanthocyanidins (PACs) chemical classes.

Phenolics containing feedstocks can include, but are not limited to, forexample, fruits from plants of the genus Vaccinium, cranberries (e.g.,juice, seeds, skin, pulp, and leaves), the juice, seeds, and skins ofgrapes, apples, fruit of locusts, cowberry fruit, bilberry, blueberry(and juice obtained therefrom), lingonberry, huckleberry, black current,chokeberry, black chokeberry, and pine bark, peanuts (e.g., peanutskins), ginkgo (e.g., ginkgo leaves), cola nuts, Rathania (e.g.,Rathania roots), cinnamon, cocoa, black tea, and green tea. In someinstances, feedstock can include, but is not limited to, (1) wholecranberries, (2) cranberry skins, (2) cranberry seeds, (3) cranberrypulp, (4) cranberry leaves, (5) whole cranberry plants, (6) and anycombination of (1)-(6). In addition, materials other than the disclosedcan be used in the processes disclosed herein if the material containsphenolics. Methods for identifying and quantifying phenolics inmaterials or feedstocks are known in the art and include, but are notlimited to, for example, direct spectroscopy at 280 nm; indirectspectroscopy using, e.g., art recognized and commercially availablereagents and assays, e.g., Vanillan assay, Folin-Denis assay,Folin-Ciocalteu assay, Prussian Blue assay, Bate-Smith assay, and Porterassay; and liquid chromatography, e.g., using ultraviolet, fluorescence,mass spectroscopy, and nuclear magnetic resonance (NMR). Phenolicsdetection techniques are also disclosed in the literature (see, e.g.,Fereidoon and Naczk, Food Phenolics, Technomic Publishing Co. Inc,1995).

Feedstock can be solid or liquid and fresh or frozen. Solid feedstockscan be liquefied or solubilized, e.g., put into solution, prior tocommencing the process. Frozen feedstocks can be thawed, e.g., prior touse. Feedstock can also be used with or without modification. Exemplaryuseful modifications can include selection, refinement, and/ormechanical processing. For example, the materials can be cleaned toremove debris (e.g., material that does not contain PACs), e.g., debris,and/or sorted to select material of a defined size. When the material isfruit (e.g., cranberry), the material can be cut into slices, and/orskinned to expose the inner flesh of the fruit (e.g., the cranberrypulp) and to increase the surface area of the material. In some cases,skins and pulp can then be used together or can be separated and usedseparately.

Feedstock can also include juice (e.g., cranberry juice) produced bytraditional pressing, enzymatic digestion, and/or by countercurrentextraction (CCE) (CCE is described in U.S. Pat. Nos. 5,320,861 and5,419,251, which are hereby incorporated by reference in theirentirety)), or juice as described in or as obtained using the methodsdescribed in U.S. Pat. Nos. 6,733,813; 6,977,092; and 7,022,368, each ofwhich is hereby incorporated by reference in its entirety.

As used herein, juice refers, e.g., to the liquid expressed or extractedfrom one or more of the fruits or vegetables disclosed in the paragraphsabove (e.g., cranberries) or a puree of the edible portions of a fruitor vegetable that is used as a beverage.

In some embodiments, feedstock is not an ultrafiltrate and/or is notpretreated using ultrafiltration, e.g., ultrafiltration, e.g., asdisclosed in U.S. Publication No. 20090035432.

The volume of liquid feedstock used in the process (e.g., in a singlecycle of the process) can be varied as required. For example, the volumeof feedstock in a single cycle of the process can include from about 100mL to about 10 gallons for small scale runs, and about 100 gallons toabout 1000 gallons, and up to, e.g., 20,000 gallons for large scaleruns.

In some instances, the volume and/or concentration of liquid feedstockused can be based upon the adsorbant capacity of the resin, which can bebased on the volume of resin present in the column), such that thevolume and/or concentration of liquid feedstock is optimized to notexceed the adsorbant capacity of the resin. For example, the volume ofliquid feedstock can be, less than, equal to, or greater than theadsorbant capacity of the resin.

The volume of liquid feedstock can be increased or decreased to providea chosen concentration of solids. In some instances, the volume of afirst feedstock with a first concentration of solids can be increased ordecreased to provide a second feedstock with more or less concentratedsolids. In some instances, the concentration of solids in a liquidfeedstock can be selected to provide a certain viscosity, e.g., suchthat the liquid feedstock allows certain flow rates. Exemplaryconcentrations of solids that can be present in a liquid feedstockinclude, but are not limited to, about 1-10%, 11-20%, 21-30%, 31-40%,41-50%, over 50%, or 25% (the concentration of solids in a liquidfeedstock can also be shown in terms of percent or Brix). Methods forincreasing the volume of a liquid feedstock include, for example, addinga volume of a suitable solution (e.g., water) to the liquid feedstock,e.g., to increase the total volume of the liquid feedstock and therebyreduce the concentration of solids in the feedstock.

Methods for decreasing the volume of a liquid feedstock include, forexample, reverse osmosis, or evaporation, or both, e.g., to decrease thetotal volume of the liquid feedstock and thereby increase theconcentration of solids in the feedstock. In some instances, the volumeof liquid feedstock can be about 346 gallons and the concentration ofsolids in the liquid feedstock can be about 50%, e.g., per column, percycle. If required, the volume of the feedstock can be increased toprovide a liquid feedstock containing about 25% solids prior tocontacting the liquid feedstock with the resin, e.g., by adding an equalvolume (e.g., about 346 gallons) of liquid (e.g., water) to the liquidfeedstock.

In some embodiments, preparation of a feedstock for use in the processesdisclosed herein does not include an extraction step, e.g., an acid oralkaline extraction step.

The size (e.g., volume or capacity or area (e.g., in m²) within a singlecolumn) of a column for use in the above disclosed process can be variedaccording to the scale of the process. For example, a small scaleprocess (e.g., a laboratory scale process) can use columns (e.g., one ormore, e.g., 2, 3, 4, 5, 10, or 20) with a capacity from about 100 mL toabout 10 gallons (e.g., 1 liter), and a large scale process (e.g., anindustrial or commercial scale process) can use columns (e.g., one ormore columns, e.g., 2, 3, 4, 5, 6, 7, 8, 9, 10, 20, or more than 20columns) with a capacity from about 10 gallons to about 1000 gallons(e.g., about 141 gallons).

The volume of liquid (e.g., water) that can be used to remove or dilutemedia used to store resin in the resin columns can include any volume ofliquid that is sufficient to completely replace the media or dilute themedia by at least about 10% (e.g., at least about 20%, 50%, 80%, 90%,95%, or 99%).

Resin suitable for use in the processes disclosed herein include, forexample, a resin (e.g., a synthetic resin) that can bind phenolicspresent in a phenolics containing feedstock. Such resins can include,for example, resin with (1) a first binding affinity for, and that binds(e.g., that binds specifically) phenolics; and (2) a second bindingaffinity for organic acids and/or sugars, wherein the second bindingaffinity is lower than the first binding affinity, e.g., such that theresin does not bind (e.g., does not substantially bind), organic acidsand/or sugars in a feedstock.

In some instances, the resin can (i) bind to non-polar to mediumpolarity phenolics, (ii) be styrene-based having one or more brominesubstituents, (iii) be hydrophobic, be a nonionic aliphatic acrylicpolymer, (iv) provide a large binding surface area, (v) be an organicresin, (vi) be an ion-exchange resin, (vii) be an aromatic resin, (viii)be a (meth)acrylic acid resin, (ix) be a (meth)acrylate resin, (x) be aacrylonitrile aliphatic resin, and/or any combination of (i)-(x).

Examples of commercially available resins that can be used in theprocesses disclosed herein include, but are not limited to, SP207Sepabeads™ (Mitsubishi Chemical), SP700 Sepabeads™ (MitsubishiChemical), Diaion HP20 (Mitsubishi Chemical), Diaion SP70 (MitsubishiChemical), Diaion SP825 (Mitsubishi Chemical), Diaion SP850 (MitsubishiChemical), Diaion HP2MG methacrylate (Mitsubishi Chemical), ADS-5(Nankai University, Tianjin, China), ADS-17 (Nankai University, Tianjin,China), Amberlite™ XAD-4 (manufactured by Organo Co. and distributedglobally by Rohm & Hass), Amberlite™ XAD-16 (manufactured by Organo Co.and distributed globally by Rohm & Hass), Amberlite™ XAD-1600(manufactured by Organo Co. and distributed globally by Rohm & Hass),Amberlite™ XAD-2 (manufactured by Organo Co. and distributed globally byRohm & Hass), Amberlite™ XAD-1180 (manufactured by Organo Co. anddistributed globally by Rohm & Hass), Amberlite™ XAD-2000 (manufacturedby Organo Co. and distributed globally by Rohm & Hass), Amberchrom™CG300-C (Rohm & Hass), and any combination thereof. In some embodiments,the resin is not the commercially available C18 resin.

In some embodiments, the resin is commercially available Amberlite™FPX66 (Rohm & Hass). FPX66 has the following properties: FPX66 consistsof white beads that form a matrix consisting of a macroreticulararomatic polymer. The moisture holding capacity of the resin is 60-68%and its specific gravity is 1.015 to 1.025. The resin has a uniformitycoefficient of less than or equal to 2.0, a harmonic mean size of0.600-0.750 mm, a fine content of less than 0.300 mm, and a surface areaof greater than or equal to 700 m²/g. The porosity of the resin is 1.4cc/g.

In some embodiments, the resin is commercially available Amberlite™XAD-7HP resin (manufactured by Organo Co. and distributed globally byRohm & Hass). Information on XAD-7HP can be found at the Rohm & Hassworld wide web site amberlyst.com/xad7hp_typical.htm. Specifically,XAD-7HP resin has the following properties: XAD-7HP is a macroreticularaliphatic crosslinked polymer ester resin that consists of whitetranslucent beads that have a moisture holding capacity of 61-69%. Theresin has an high surface area (e.g., approximately or about 300-500m²/g (e.g., greater than 380 m²/g)), a specific gravity of 1.06 to 1.08,an average pore size of approximately 450 Angstroms, a mean diameter ofapproximately 560 μm, and both a continuous polymer phase and acontinuous pore phase. The harmonic mean size of the beads is 0.56-0.71mm with a uniformity coefficient of less than or equal to 2.0. Themaximum operating temperature of the resin is 80-100° C. (i.e., 175-210°F.).

The amount of resin used can be varied and is dependent upon the scaleof the process and/or the volume or capacity of the column.

The time and conditions sufficient for phenolics present in thefeedstock to bind to the resin include those times and conditions underwhich at least and/or about 1% or 10% (e.g., at least and/or about 1%,5%, 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 95%, 99% and 100%, or arange between any two of these values) of phenolics present in theliquid feedstock bind to the resin. Methods for assessing the percentageof phenolics present in the feedstock that have bound to the resin caninclude, for example, steps of first assessing the level of phenolics inthe liquid feedstock, and then assessing the level of phenolics presentin the feedstock flow through and/or the non-phenolics containingpermeate, wherein any difference in the level of phenolics is anindication of the level of phenolics bound to the resin. Methods fordetecting phenolics are known in the art and are exemplified above.

The time and conditions sufficient for phenolics present in thefeedstock to bind to the resin can be controlled, e.g., by varying theflow rate of the feedstock into the resin (e.g., the time the feedstockis contacted with the resin), and/or the temperature within the resincolumn. For example the flow rate of the feedstock into the resin columncan include, but is not limited to, about 1.0-6.0 gallons per minute(e.g., about 1.0, 1.5, 2.0, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9,3.0, 3.1, 3.2, 3.3, 3.4, 3.5, 3.6, 3.7, 3.8, 3.9, 4.0, 4.1, 4.2, 4.3,4.4, 4.5, 4.6, 4.7, 4.8, 4.9, 5.0, 5.5, 6.0 gallons per minute), and thetemperature within the resin column can be selected to minimizemicrobial growth (e.g., 35° F.-80° F.).

As noted above, a volume of feedstock flow through can be discarded aswaste or redirected for further processing. Exemplary volumes that canbe discarded include at least and/or about, 0.5%, 1%, 5%, 10%, and about20%.

Wash solutions useful in the processes disclosed herein can include, forexample, water based solutions containing one or more solvents that willnot reduce (e.g., substantially reduce) the amount of phenolics bound tothe resin. Exemplary wash solutions can include water or water mixedwith one or more solvents, for example, water containing up to about 25%solvent (e.g., up to and/or about 25%, 20%, 15%, 10%, 5%, 4%, 3%, 2%,1%, 0.5%, and/or below 0.5% solvent). Suitable solvents include, but arenot limited to, e.g., alcohol (e.g., methanol, ethanol, propanol),acetone, hexane, and/or mixtures thereof.

In some instances, the wash solution is a water based solutioncontaining 5%±1% total ethanol (e.g., 1 part ethanol in 19 parts water).The volume of wash solution can be adapted to the volume or capacity ofthe resin-containing column, wherein one-times the volume of resin inthe column is referred to as one bed volume. For example, the volume ofwash solution can include, e.g., less than one bed volume, about one bedvolume, about two bed volumes, or more than two bed volumes.

In some instances, the volume of wash solution can be about 282 gallonsor about 2 bed volumes and the wash solution can include about 5%±1%total ethanol (e.g., 1 part ethanol in 19 parts water).

In some instances, the volume of wash solution can be about 282 gallonsor about 2 bed volumes and the wash solution can include about 5%±1%Standard Denatured Alcohol (SDA), e.g., SDA 35A 190, in water (furtherinformation regarding SDA 35A 190 can be found at world wide web addresssasoltechdata.com/tds/sda35A_(—)190.pdf).

A single cycle can include 1 or more wash steps (e.g., 1, 2, 3, 4, 5, ormore wash steps) per each cycle. Furthermore, the wash steps can beperformed for a time and under conditions that allow optimal removal ofnon-phenolics from the resin. For example the flow rate of the washsolution into the resin column can include, but is not limited to,1.0-6.0 gallons per minute (e.g., about 1.0, 1.5, 2.0, 2.1, 2.2, 2.3,2.4, 2.5, 2.6, 2.7, 2.8, 2.9, 3.0, 3.1, 3.2, 3.3, 3.4, 3.5, 3.6, 3.7,3.8, 3.9, 4.0, 4.1, 4.2, 4.3, 4.4, 4.5, 4.6, 4.7, 4.8, 4.9, 5.0, 5.5,6.0 gallons per minute), and the temperature within the resin column canbe selected to minimize microbial growth (e.g., 35° F.-80° F.).

Elution solutions that can be used in the processes disclosed herein caninclude water based solutions containing one or more solvents at anyconcentration that will decrease (e.g., substantially decrease) theassociation between resin-bound phenolics and resin, such that phenolicsare released from the resin. Exemplary elution solutions can includesolvent or a mixture of solvent and water (e.g., wherein theconcentration of the solvent is about 100%, or less than 100%, e.g.,about 99%, 98%, 97%, 96%, 95%, 90%, 85%, 80%, 75%, 70%, 60%, 55%).Suitable solvents can include, but are not limited to, e.g., alcohol(e.g., methanol, ethanol, propanol), acetone, and hexane. In someinstances, the elution solution is a water based solution containing95%±1% total ethanol. The volume of elution solution can include, e.g.,less than one bed volume, about one bed volume, about two bed volumes,or more than two bed volumes.

In some instances, the volume of elution solution can be about 346gallons or about 2.8 bed volumes and the elution solution can include95% total ethanol in water.

In some instances, the volume of elution solution can be about 346gallons or about 2.8 bed volumes and the elution solution can include95% Standard Denatured Alcohol (SDA), e.g., SDA 35A 190, in water.

A single cycle can include 1 or more elution steps (e.g., 1, 2, 3, 4, 5,or more elution steps) per each cycle. Furthermore, the elution stepscan be performed for a time and under conditions that allow optimalremoval of phenolics from the resin. For example the flow rate of thewash solution into the resin column can include, but is not limited to,1.0-6.0 gallons per minute (e.g., about 1.0, 1.5, 2.0, 2.1, 2.2, 2.3,2.4, 2.5, 2.6, 2.7, 2.8, 2.9, 3.0, 3.1, 3.2, 3.3, 3.4, 3.5, 3.6, 3.7,3.8, 3.9, 4.0, 4.1, 4.2, 4.3, 4.4, 4.5, 4.6, 4.7, 4.8, 4.9, 5.0, 5.5,6.0 gallons per minute), and the temperature within the resin column canbe selected to minimize microbial growth (e.g., 35° F.-80° F.).

In some embodiments, elution flow through is collected without furtherprocessing. In other embodiments, elution flow through is furtherprocessed. For example, phenolics containing solutions can be evaporatedusing at least one evaporation step (e.g., 1, 2, 3, 4, 5, or moreevaporation steps), e.g., to reduce the amount of solvent present in thesolution, resulting in a solution containing phenolics, water, and areduced amount of solvent as compared to the elution solution. In someinstances, the evaporation step can be repeated to further reduce theamount of solvent present in the solution. Evaporation methods caninclude, but are not limited to, e.g., batch evaporation methods andcontinuous evaporation methods, falling film methods, rising filmmethods, falling plus rising film methods (e.g., using plates andtubes), multiple effects methods, single effects methods, and vaporrecompression.

In some instances, the evaporation step can be repeated, e.g., until theamount of solvent in the solution is less than about 90 parts permillion. Evaporation methods can include, for example, the use oftemperature (e.g., heat) and/or pressure (e.g., vacuum) sufficient toreduce the amount of solvent in solution. Exemplary conditions caninclude a temperature of at least about 70° C. (e.g., about 71° C., 72°C., 73° C., 74° C., 75° C., 76° C., 77° C., 78° C., 79° C., 80° C., 81°C., 82° C., 83° C., 84° C., 85° C., 86° C., 87° C., 88° C., 89° C., 90°C. and above 90° C. (or the Fahrenheit equivalent)) and pressure of atleast about 50 mBar (e.g., 50 mBar, 60 mBar, 70 mBar, 80 mBar, 90 mBarand above 90 mBar). In some embodiments, evaporation conditions can beabout 124° F. and about 130 mBar. Other exemplary evaporation conditionsinclude combinations of temperature and vacuum shown in Table 1.

TABLE 1 Exemplary Evaporation Conditions Temperature (° F.) Vacuum(mBar) 104 73.77 114 98.61 124 130.35 134 170.46 144 220.67 154 282.93164 359.48 174 452.8 184 565.72 194 701.24 204 862.9 212 2605.4

In some instances, an evaporation step can be carried out in conjunctionwith a step to reduce the concentration of solids in the liquid (e.g., adilution step).

In some embodiments, evaporation can include (i) removing solvent fromthe phenolics containing solutions and (ii) concentrating the phenolicscontaining solutions. In some instances, (i) and (ii) are performedsimultaneously using e.g., a rotary evaporator (Rotovap). Alternatively,(i) can be performed using, e.g., distillation columns and (ii) can beperformed either simultaneously or subsequently. Other exemplary methodsfor removing solvent include, but are not limited to, the use oftemperature and/or vacuum as described above using, e.g., a rotaryevaporator (Rotovap).

In some embodiments, evaporation can include (i) removing solvent fromthe phenolics containing solution using distillation columns and (ii)concentrating the phenolics containing solutions using a rising filmplate evaporator. Alternatively or in addition, evaporation can includethe use of forced circulation evaporators, or Pfaudler kettles.

The solution resulting from the evaporation step is a liquid (e.g.,water) containing phenolics (a liquid extract). This extract does notcomprise fruit juice, e.g., fruit juice expressed or extracted from afeedstock disclosed above in the absence of the process disclosedherein; or fruit juice produced by traditional pressing, enzymaticdigestion, or by CCE; or juice as described in or as obtained using themethods described in U.S. Pat. Nos. 6,733,813; 6,977,092; and 7,022,368.In some instances, the liquid extract can be obtained and optionallyanalyzed, e.g., to assess the level of phenolics and/or to characterizethe types of phenolics present.

The liquid extract can be concentrated, e.g., to increase theconcentration of solids in the extract. Concentration methods include,but are not limited to, e.g., one or more of, membrane concentration,heat concentration, vacuum (reduced pressure) concentration, and freezeconcentration. In some instances, this liquid extract can be obtainedand optionally analyzed, e.g., to assess the level of phenolics and/orto characterize the types of phenolics present.

Liquid extracts can be dried to provide a dry extract containingphenolics. Methods for drying the liquid extracts can include, but arenot limited to, for example, freeze drying, vacuum drying, spray drying,drum drying, shelf drying, and drying by microwave.

If required, a liquid or dry extract can be analyzed, e.g., to assessthe level of phenolics present and/or to characterize the phenolicspresent (e.g., to determine the relative amounts of phenolics (e.g.,anthocyanins and PACs) present in the extract.

Extracts can also be optionally sterilized. Sterilization can beperformed by a method commonly used by those skilled in the art, such ashigh-pressure sterilization, heat sterilization, filter sterilization,and microwave sterilization.

Extracts

The extracts (e.g., phenolics enriched extracts) obtained using theprocesses disclosed herein can be liquid, dry, semi dry, or powderedextracts (e.g., powdered, dehydrated, or lyophilized extracts)containing at least anthocyanins and proanthocyanidins (PACs). Suchextracts can be additionally characterized as having or containing atotal amount of anthocyanins of at least about 1% (weight to volume(w/v), weight to weight (w/w), or volume to volume (v/v)), as assessed(e.g., quantified) using HPLC. For example, extracts can contain atleast about or about 1%, e.g., at least about 2%, 3%, 4%, 5%, 6%, 7%,8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20% (w/v, w/w,or v/v), or at least about 21% (w/v, w/w, or v/v), or a range betweenany two of these values, anthocyanins, as assessed by HPLC. Suchextracts can also contain at least about 10% (w/v, w/w, or v/v) PACs, asassessed (e.g., quantified) using, e.g., HPLC. For example, extracts cancontain at least about or about 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%,50%, 55%, 60% (w/v, w/w, or v/v), more than 61%, 65%, 70%, 75%, or atleast about 80% (w/v, w/w, or v/v), or a range between any two of thesevalues, PACs, as assessed by HPLC.

The levels of PACs in an extract can be assessed or quantified usingDMAC (the DMAC method is disclosed in Cunningham et al., Analysis andStandardization of Cranberry Products, Quality Management ofNutraceuticals, ACS Symposium Series, 803ed., American Chemical Society,Washington D.C., pages 151-166, 2002, which is hereby incorporated byreference). In such instances, extracts containing at least anthocyaninsand proanthocyanidins (PACs) can contain about or at least about 40%(w/v, w/w, or v/v) PACs, as assessed (e.g., quantified) using, e.g.,DMAC. For example, extracts can contain at least about or about 40%,50%, 55%, 60%, 70%, 80% (w/v, w/w, or v/v), more than 80% (w/v, w/w, orv/v), or a range between any two of these values PACs, as assessed byDMAC; and/or

a ratio of anthocyanins to PACs of about 1:5 (e.g., about 1:2, 1:2.5,1:3, 1:3.5, 1:4, 1.4.5, 1:5, 1:5.5, 1:6, 1.6.5, 1:7, 1:7.5, 1:8, 1:8.5,1:9, 1:9.5, or about 1:10), wherein anthocyanins are assessed (e.g.,quantified) using, e.g., HPLC, and PACS are assessed (e.g., quantified)using, e.g., HPLC or DMAC; and/or

a PACs oligomeric profile that substantially the same or similar, (e.g.,substantially similar) to the PACs oligomeric profile present in CCEcranberry juice feedstock. Alternatively or in addition, the PACsoligomeric profile can include higher amounts of 2-mer and greater than10-mers than other PACs oligomers. Alternatively or in addition, thePACs oligomeric profile can include ratios of PACs oligomers of about6(1mer):28(2mer):11(3mer):8(4mer):6(5mer):7(6mer):3(7mer):4(8mer):2(9mer):26(>10mer); and/or

a ratio of PACs to total phenolics that is substantially the same (e.g.,roughly equal) to the ratio of PACs to total phenolics present incranberries or the fruit from which the phenolics were extracted, e.g.,present in cranberries or counter current extracted cranberry juice;and/or

a ratio of PACs to quercetin, quercgalac, quercitrin, myricetin, and/orquercaraban that is the same (e.g., substantially the same) or similar,(e.g., substantially similar) to the ratio of PACs to quercetin,quercgalac, quercitrin, myricetin, and/or quercaraban present in CCEcranberry juice; and/or

a ratio of PACs to total anthocyanins that is not the same as the ratioof PACs to total anthocyanins in cranberries or the fruit from which thephenolics were extracted, e.g., present in cranberries or countercurrent extracted cranberry juice; and/or.

phenolics (e.g., polymeric phenolics) with a molecular weight (e.g., anaverage molecular weight) of less than 14,000 Daltons; and/or

PACs (e.g., 10% or more of total PACs in the extract) with polymer chainlengths of 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, or greater than 10, orcombinations thereof; and/or

a higher concentration of anthocyanin and PACs than is present incranberries or the fruit from which the phenolics were extracted, e.g.,present in cranberries or counter current extracted cranberry juicefeedstock, e.g., a higher dry weight concentration.

Extracts containing at least anthocyanins and proanthocyanidins (PACs)can be optionally further characterized based on the levels of organicacids (e.g. total organic acids) and sugars (e.g., total sugars) in theextract. For example, extracts can contain less than 5% (w/v, w/w, orv/v) organic acids (e.g., about 5% or less than about 5%, 4%, 3%, 2%, 1%organic acids, less than 1% organic acids, no organic acids (e.g., theextract can be free (e.g., substantially free) of organic acids), or arange between any two of these values), and/or less than 5% sugar (e.g.,about 5% or less than about 5%, 4%, 3%, 2%, 1% sugar, less than 1%sugar, no sugar (e.g., the extract can be free (e.g., substantiallyfree) of sugar), or a range between any two of these values).

The phenolics extracted using the process described herein can besoluble in aqueous media.

An extract can be formulated as a composition for use in an animal(e.g., a human and/or non-human animal), e.g., for ingestion orconsumption by an animal (e.g., a human and/or non-human animal). Suchcompositions can include excipients, e.g., to increase the stability,solubility, shelf-life, taste, to standardize the level of a particularcompound in the composition, and/or bioabsorption of the extract.Examples of includable excipients include but are not limited to,calcium carbonate, calcium phosphate, various sugars and types ofstarch, cellulose derivatives, gelatin, vegetable oils, polyethyleneglycols, propylene glycol, and inhibitors of enzymes that degrade and/ormodify phenolics, such as inhibitors of polyphenoloxidases, peroxidases,glycosidases, decarboxylases, and esterases. Alternatively or inaddition, the extracts can be combined with agents that protect themfrom oxidative reactions (e.g., anti-oxidants). Different diafiltrationmedia (e.g., acidified water) can be employed to stabilize and/or adjustthe color of the extract

Use of Extracts

In some embodiments, the extracts disclosed herein can be used in or asnutriceuticals and/or as food supplements. For example, the extracts canbe formulated to as powders, pills, tablets, capsules or syrups foradministration to an individual (e.g., a human or non-human) by anyroute, e.g., by ingestion. Alternatively or in addition, the extractscan be used to supplement a food or beverage to enhance the healthbenefits conferred by the food or beverage. For example, such an extractcould be applied to (e.g., coated onto or infused into) fruits,vegetable, legumes, and the like (e.g., dried cranberries) to create afood product with enhanced health benefits. Alternatively or inaddition, extracts can be used to supplement beverages, e.g., juicebeverages including, but not limited to, e.g., fruit juices and fruitjuice drinks (e.g., cranberry juice cocktails and juice blends), tea(e.g., herbal and non-herbal tea), leaf tea, yogurt, milk, smoothies,chewing gum, dietary supplements, water, flavored waters, energy drinks,and milk (e.g., liquid and powdered milk).

Compositions Comprising Phenolics and Fumaric Acid and Uses Thereof

Species of the genus Alicyclobacillus (ACB) include, for example,acidophilic, thermophilic, and spore forming bacteria such asAlicyclobacillus acidoterrestris and Alicyclobacillus acidocaldarius.ACB contamination of juice beverages can cause spoilage due to theproduction of guaiacol, an organic compound that imparts an unpleasantflavor and odor.

ACB contamination of juice beverages can be caused by the presence ofsoil residue in the juice beverage. Accordingly, careful washing offruit with uncontaminated water during processing can reduce ACBcontamination. Some fruits, however, are difficult to wash thoroughly.Such methods are also inefficient and can not be applied to previouslyprocessed and packaged juice beverages (e.g., packaged juice). ACBcontamination can also be present in raw or refined sugar added tojuices. Once ACB is present in a production line it can be difficult toeliminate because ACB spores are heat resistant. Pasteurization cannotalways be used to eliminate ACB because the high temperatures requiredto eliminate ACB spores can be detrimental to juice quality. Certaintypes of filtration and irradiation can also be used to eliminate ACB,but such methods are not suitable for all products. High concentrationsof phenolics can reduce ACB contamination in beverages such as fruitjuice. The application of such methods are limited, however, because thelevel of phenolics required to cause an undesirable change in the colorof the beverage. Fumaric acid alone can also reduce ACB contamination injuice beverages, but not without undesirably altering the taste of thejuice.

Provided herein are compositions comprising (e.g., comprising,consisting essentially of, or consisting of) phenolics (e.g.,concentrated, isolated, or purified phenolics (e.g., the extractsdisclosed herein)) and fumaric acid. These compositions can be added tojuice beverages that are susceptible to microbial (e.g., bacterialcontamination or ACB contamination), to reduce or prevent microbial(e.g., bacterial contamination or ACB contamination) contaminationtherein.

Juice beverages susceptible to ACB contamination include, but are notlimited to, for example, juice beverages contaminated with soil, juicebeverages contaminated with raw or refined sugar containing ACB or ACBspores, and juice beverages containing ACB spores. Alternatively, thecompositions can be added to juice beverages to reduce or prevent ACBcontamination in ACB contaminated juices, e.g., juices containing viableACB microbes. As ACB contamination reduces the shelf-life of juicebeverages, the compositions described herein can be used to increase theshelf-life of juice beverages, or as juice beverages preservatives.Furthermore, such results can be achieved without undesirably alteringthe taste or appearance of the juice beverage due to the synergisticactivity between the two components. As used herein, “synergy” or“synergistic activity” and the like refer to a combined effect of twocomponents that is greater than the individual effects of the samecomponents alone or when added together. For example, as used herein,synergy refers to a level of reduction in ACB contamination of a juicebeverage, a reduction in ACB growth, or an increase in the death of ACBin the presence of fumaric acid and phenolics that is not observed inthe presence of fumaric acid or phenolics alone.

In some embodiments, the compositions and methods disclosed hereinprevent or reduce ACB growth, kill quiescent or dividing ACB cells,and/or eliminate ACB spores.

In some embodiments, compositions comprising ratios of phenolics tofumaric acid can include ratios of phenolics (e.g., PACs) to fumaricacid that are useful in higher acidity juice beverages. Exemplary higheracidity juice beverages can include, but are not limited to, apple juice(e.g., about pH 2.9-3.3 or about pH 2.9-4.1, e.g., pH 3.7), lemon juice(e.g., about pH 2.3), cranberry juice (e.g., about pH 2.3-2.5), tropicalfruit blends, grapefruit juice (e.g., about pH 2.9-3.5), pineapple juice(e.g., about pH 3.4), grape juice (e.g., about pH 2.8-3.3), and juiceblends containing two or more of these juices alone or in combinationwith low pH juice beverages. In some embodiments, higher acidity juicebeverages have a pH of between about pH 2-pH 3.49.

In some embodiments, compositions comprising ratios of phenolics tofumaric acid can include ratios of phenolics (e.g., PACs) to fumaricacid that are useful in lower pH (e.g., lower acidity) juice beverages.Exemplary low pH juice beverages can include, but are not limited to,for example, orange juice (e.g., about pH 3.5-4.2, pH 3.9, or pH 4.6)and/or vegetable juice (e.g., about pH 3.9-4.3). In some embodiments,lower pH juices have a pH of greater (i.e., more alkaline) than about pH3.5.

Useful ratios of fumaric acid to phenolics (e.g., PACs) can include, butare not limited to, e.g., ratios of fumaric acid to PACs of betweenabout 4000:1-100:1, 3571:1-121:1, about 135:1, or about 238:1. In someinstances, such ratios can be useful in higher acidity juice beverages.

Other useful ratios of fumaric acid to phenolics (e.g., PACs) for use inhigh pH juice beverages can include, but are not limited to, e.g.,ratios of fumaric acid to PACs of between about 10:1-50:1, or 14:1. Insome instances, such ratios can be useful in lower pH juice beverages.

In some instances, phenolics and fumaric acid can be present in a juicebeverage at synergistic concentrations. With respect to fumaric acid,such concentrations can be defined using any art recognized units (e.g.,percent weight/volume (e.g., g/100 mL) or percent volume/volume).Concentrations of phenolics can also be defined using any art recognizedterm (e.g., percent weight/volume (e.g., g/100 mL) or percentvolume/volume) and can be expressed either as total phenolics or byspecific phenolics (e.g., proanthocyanidins (PACs)). In someembodiments, phenolics can be extracts obtained using the processesdisclosed herein and the amount of PACs in the extract can be about 55%.

In some embodiments, concentrations of added phenolics and fumaric acidin a beverage can include, e.g., 4.2×10 mg/mL PACs (i.e., 0.1 mg PACs/8oz)-8.291×10⁻³ mg/mL PACs (i.e., 1.99 mg PACs/8 oz), 2.1×10⁻³ mg/mLPACs-8.3×10⁻³ mg/mL PACs, 2.1×10⁻³ mg/mL PACs-6.3×10⁻³ mg/mL PACs, orabout 4.2×10⁻³ mg/mL PACs (i.e., 1 mg PACs/8 oz), and between about0.01% (e.g., 0.001%-0.05%) to about 0.15% (e.g., about 0.10%-0.2%)fumaric acid, or about 0.1% fumaric acid by weight (e.g., w/w if theextract and fumaric acid are dry, v/w, or vice-versa, if one of theextract or liquid is dry).

In other embodiments, concentrations of added phenolics and fumaric acidin a beverage can be, e.g., 4.2×10⁻⁴ mg/mL PACs (i.e., 0.1 mg PACs/8oz)-100 mg/mL PACs, 4.2×10⁻⁴ mg/mL PACs-80 mg/mL PACs, 4.2×10⁻⁴ mg/ml,PACs-60 mg/mL PACs, 4.2×10⁻⁴ mg/mL PACs-40 mg/mL PACs, 4.2×10⁻⁴ mg/mLPACs-20 mg/mL PACs, 4.2×10⁻⁴ mg/mL PACs-10 mg/mL PACs, 4.2×10⁻⁴ mg/mLPACs-5 mg/mL PACs, 4.2×10⁻⁴ mg/ml PACs-1 mg/mL PACs, 4.2×10⁴ mg/mLPACs-0.5 mg/mL PACs, 4.2×10⁻⁴ mg/mL PACs-0.1 mg/mL PACs, 4.2×10⁻⁴ mg/mLPACs-0.05 mg/mL PACs, 4.2×10⁻⁴ mg/mL PACs-0.04 mg/mL PACs, 4.2×10⁻⁴mg/mL PACs-0.03 mg/mL PACs, 4.2×10⁻⁴ mg/mL PACs-0.02 mg/mL PACs,4.2×10⁻⁴ mg/mL PACs-0.01 mg/mL PACs, 4.2×10⁻⁴ mg/mL PACs-0.005 mg/mLPACs, 4.2×10⁻⁴ mg/mL PACs-0.001 mg/mL PACs, or 0.05 mg/mL PACs (i.e., 12mg PACs/8 oz), and between about 0.01% (e.g., 0.001%-0.05%) to about0.15% (e.g., about 0.10%-0.2%) fumaric acid, or about 0.1% fumaric acidby weight (e.g., w/w if the extract and fumaric acid are dry, v/w, orvice-versa, if one of the extract or liquid is dry).

In some embodiments, concentrations of phenolics and fumaric acid caninclude, but are not limited to, for example, no more than about 0.04mg/ml PACs, or no more than about 1 mg/ml PACs, or about 0.04 mg/ml toabout 0.17 mg/ml (e.g., 0.15 mg/ml-0.19 mg/ml) PACs, and about 0.01%(e.g., 0.001%-0.05%) to about 0.15% (e.g., about 0.10%-0.2%) fumaricacid by weight.

In some embodiments, compositions comprising phenolics and fumaric acid(e.g., dry phenolics and fumaric acid) can be prepared in amounts thatare sufficient to yield synergistic concentrations of phenolics andfumaric acid when added to a volume of juice beverage. Such compositionscan be prepared according to any of the above ratios alone or in amountssufficient to provide a synergistic concentration of phenolics andfumaric acid when the composition is added to a defined volume of ajuice beverage. Such compositions are within the present invention.Exemplary volumes of juice beverage to which such compositions can beprepared and/or added include, but are not limited to, 0.1, 0.5, 1, 10,20, 50, 100, 200, 250, 300, 330, and 500 mLs, 1, 2, 2.5, 5, 10, 15, 20,30, 50, 100, 150, 200, 250, 500, 750, 1000, 10,000, 25,000, 50,000,100,000, 500,000, 1000,000 L, and above 1000,000 L (or the equivalentvolumes in ounces and gallons), or a range between any two of theafore-listed integers.

Methods of Making Compositions Comprising Phenolics and Fumaric Acid

Compositions comprising phenolics and fumaric acid can includeconcentrated, purified, or isolated phenolics that include at leastPACs, wherein the phenolics are concentrated and/or isolated from anyphenolics containing feedstock, e.g., any phenolics containing feedstockdisclosed herein. Methods for concentrating and/or isolating phenolicsare known in the art and include, but are not limited to, for example,filtration. and those methods disclosed in, for example, U.S. Pat. Nos.5,840,322, 6,440,471, 6,210,681, 5,650,432, 5,646,178, 5,474.774,5,525,341, 6,720,353, and 6,608,102.

In some embodiments, phenolics are the extracts disclosed here or areobtained using the processes disclosed herein.

In some embodiments, extracts for use in or as food preservativescontain about 90% PACs (e.g., type A PACs).

Fumaric acid, as included in the compositions disclosed herein, caninclude any commercially available fumaric acid and the salts and estersthereof (e.g., fumarates). Fumaric acid is also referred to in the artas trans-butenedioic acid, has the chemical formula HO₂CCH═CHCO₂H, andhas a molecular mass of 116.07 g/mol. Compositions comprising phenolicsand fumaric acid can be prepared using any combination of liquid or dryphenolics and fumaric acid. Similarly, compositions comprising phenolicsand fumaric acid can themselves be liquid or dry (e.g., spray dried). Insome embodiments, compositions comprising phenolics and fumaric acid aredry (e.g., spray dried). In some embodiments, compositions comprisingphenolics and fumaric acid can include isolated phenolics and isolatedacid. Such compositions can also consist of or consist essentially ofisolated phenolics and isolated fumaric acid. The compositions can becontained in any suitable container, vessel or vial suitable for storingor distributing the composition and/or adding the composition to a juicebeverage. For example, compositions can be disposed within a containerin amounts sufficient to yield synergistic concentrations of phenolicsand fumaric acid when the composition is added to a liquid volume ofjuice beverage. Exemplary suitable containers include, but are notlimited plastic, glass, metal, and paper vessels suitable for single useor multiple use. In some embodiments, containers can be marked orlabeled to illustrate either the amount of the composition containedtherein or that volume of juice beverage to which the composition shouldbe added, e.g., to yield a synergistic concentration of phenolics andfumaric acid. For example, a container containing sufficient levels oramounts of phenolics and fumaric acid to provide synergisticconcentrations of phenolics and fumaric acid in 1 L of juice beveragecan be marked or labeled “1 L.”

EXAMPLES

The invention is further described in the following examples, which donot limit the scope of the invention described in the claims.

Example 1 Extract Characterization (Small Scale Extraction)

Extract obtained using small scale (laboratory scale) processes wascharacterized to determine the types of phenolics present and therelative amounts of the different phenolics present (i.e., the relativeamounts of one type of phenolic to another type of phenolic).Non-phenolic material was also assessed.

Briefly, feedstock (i.e., counter current extraction (CCE) cranberryjuice) was concentrated using reverse osmosis to increase the Brixcontent from 1 Brix (the concentration obtained following CCE) to 18Brix and evaporation to further increase the Brix content from 18 Brixto 50 Brix. The 50 Brix CCE feedstock was then diluted in water to 25Brix. Diluted feedstock was contacted with Amberlite™ XAD-7HP resin.Flow through was collected as reduced-phenolics permeate. Resin waswashed using 5% SDA ethanol wash solution. Flow through was collected aswash solution flow through. Bound phenolics were then eluted using 95%SDA ethanol elution solution. Elution solution flow through wascollected and concentrated using evaporation (heat and vacuum) toproduce a liquid extract containing 25% solids. Extract was then spraydried using a NIRO mobile minor spray drier Volumes used in the abovesmall scale process are shown in Table 2.

TABLE 2 Volumes Used in Single Cycle of Small Scale Process Small(Laboratory) Scale (mL unless shown) Column Volume 1 L Volume of Resinin Column 943 Volume of feedstock (50 Brix) 2452 Volume of Feedstock (25Brix) 4904 Volume of feedstock not collected 709 as feedstockflowthrough Volume of reduced- 4195 phenolics feedstock flow throughVolume of Wash Solution 2000 Volume of Elution Solution 2452 Flow rate(all steps) 15.6 mL/minute Re-equilibration volume 2678

Distinct extracts from multiple small scale single cycle runs wereanalyzed to determine the levels of phenolics, organic acids, andsugars. Data from each run was combined and means calculated. Theresults of these experiments are shown in Table 3.

TABLE 3 Extract Characterization Av. (%) Min. (%) Max. (%) Std. Dev. %Solids 95.52 95.18 97.25 0.97 Quinic 0.07 0.03 0.12 0.05 Malic 0.17 0.110.25 0.07 Citric 0.26 0.11 0.38 0.11 Total Organic Acids 0.50 0.39 0.760.17 PACs ² 55.00 55.00 55.00 0.00 PACs ¹ 18.20 18.20 18.20 0.00Phenolics (Folin) 44.40 35.49 55.44 8.50 Anthocyanins ¹ 6.86 4.94 9.782.24 Dextrose 0.17 0.00 0.67 0.33 Fructose 0.00 0.00 0.00 0.00 Sucrose0.00 0.00 0.00 0.00 Total Sugars 0.17 0.00 0.67 0.33 Quercetin 0.54 0.171.22 0.59 Quercitrin 3.03 0.95 4.23 1.81 Hyperoside 2.24 0.93 4.71 2.14Myricetin 0.24 0.09 0.52 0.24 Rutin 0.00 0.00 0.00 0.00 Kaempferol 0.000.00 0.00 0.00 Isorhamnetin 0.00 0.00 0.00 0.00 Isoquercitrin 0.00 0.000.00 0.00 Total Flavonols 6.05 5.17 7.06 0.95 Standardizing carrier20.58 9.50 32.10 12.57 Flow Agent 0.80 0.80 0.80 0.00 Total Recovery121.44 101.18 144.77 ¹ Assessed by HPLC ² Assessed by DMAC ^(1 and 2)are normalized values

As shown in Table 3, the primary component of extract is phenolics (see“Phenolics (Folin)” in Table 3) with PACs and anthocyanidins present atthe highest levels. Levels of flavanols quercetin, quercitrin,hyperoside, and myricetin were also detected.

In contrast, extract is substantially free of sugars (see “Total Sugars”in Table 3) and organic acids. Moreover, total organic acid content wasless than 1% and total sugar content was less than 1%. Theseobservations indicate that the process disclosed herein can be used toobtain extract from a CCE cranberry juice feedstock that contains highamounts of PACs and anothocyanins and that is substantially free oforganic acids and sugars. Furthermore, the low standard deviation valuesshown confirm that between batch variation is low, or that the levelsshown in Table 3 can be consistently obtained.

As noted above, PACs include molecules of various chain lengths.Experiments were performed to determine the chain lengths of PACspresent in Extract. Experiments were also performed to determine thechain lengths of PACs present in multiple CCE feedstock samples used toobtain extract to allow comparison of PACs profiles in feedstock andextract. The results are shown in Table 4.

TABLE 4 PACs Oligomeric Content of Extract (gPAC Oligomers/100 g PACs)Sample 1mers 2mers 3mers 4mers 5mers 6mers 7mers 8mers 9mers10mers >10mers ID (%) (%) (%) (%) (%) (%) (%) (%) (%) (%) (%) total %CCE 1 6.87 25.31 10.35 6.10 4.27 5.11 1.46 1.59 2.39 0.00 36.55 100.00CCE 2 5.11 30.86 11.52 9.13 6.67 8.12 3.40 3.23 1.81 0.00 20.14 100.00CCE 3 7.02 29.39 9.98 7.14 5.13 6.11 3.25 3.88 2.81 0.00 25.28 100.00CCE 4 6.27 26.22 10.08 7.89 5.82 7.06 4.01 4.03 2.70 0.00 25.92 100.00CCE 5 5.52 28.95 11.38 8.40 6.19 7.53 3.99 4.15 2.75 0.00 21.15 100.00Mean 6.2 28.15 10.66 7.73 5.62 6.79 3.22 3.4 2.49 0 25.8 Extract 6.3528.23 10.71 8.08 5.56 7.06 4.00 4.21 1.93 0.00 23.87 100.00

As shown in Table 4, the PACs profile in extract is substantiallysimilar to the PACs profiles detected in multiple CCE feedstock samples.This observation suggests that the processes disclosed herein can beused to obtains PACs at levels present in a CCE feedstock. Morespecifically, 2mer and >10mer PACs are most prevalent in both extractand CCE feedstock. Levels of other PACs oligomers are also preserved inextract as compared to CCE feedstock. As revealed by these experiments,the ratios of PACs oligomers in both extract and CCE feedstock is about6(1mer):28(2mer):11(3mer):8(4mer):6(5mer):7(6mer):3(7mer):4(8mer):2(9mer):26(>10mer). Therefore, the processes disclosed herein can be used to obtain anabstract containing anthocyanins and PACs, wherein PACs oligomers arepresent at levels present in the feedstock.

Experiments were next performed to assess the levels of PACs to otherphenolics, including anthocyanins and total phenolics, in extract andfeedstock. These experiments were performed to allow determination ofwhether the ratios of PACs to other phenolics present in feedstock arepreserved in extract. The results of these experiments are shown inTables 5 and 6.

TABLE 5 Ratios of PACs and Phenolics in CCE Feedstock and Extract SampleID 1 2 3 4 Sample Type CCE Extract CCE Extract CCE Extract CCE ExtractTotal PACs (%)- dry weight 2.6 97.9 1.6 77.6 1.8 81.1 1.9 85.2 TotalPhenolics (%)- dry weight 2.2 62.5 1.6 52.6 1.8 56.1 1.7 58.5 PACs:totalPhenolics 1.3 1.6 1.2 1.5 1.1 1.4 1.3 1.4 PACs:total anothocyanin (TAcy)5.7 14.0 4.9 11.2 5.3 13.2 6.7 16.3 Total sugars- dry weight 64.1 0.070.3 0.0 69.2 0.0 66.5 0.0 Total organic acids- dry weight 40.5 0.3 48.30.3 44.5 0.3 42.9 0.3 PACs (ppm)/Quercetin (ppm): 279.7 207.2 231.4191.6 215.7 194.1 43.5 51.1 PACs (ppm)/QuercGalac (ppm): 39.1 22.3 21.913.0 18.8 15.3 28.3 24.4 PACs (ppm)/Quercitrin (ppm): 152.8 99.2 92.375.3 99.6 93.5 96.9 97.5 PACs (ppm)/Myricetin (ppm): 187.6 170.3 221.2183.1 144.7 148.7 36.3 38.8 PACs (ppm)/QuercAraban (ppm): 108.3 76.885.7 74.8 64.1 64.1 74.7 75.1 Total Solids (%) 55.4 95.7 58.4 96.0 54.797.1 56.3 97.9

TABLE 6 Average Ratios of PACs and Phenolics in CCE Feedstock andExtract Average ratios CCE Extract PACs:Phenolics 1.2 1.5 PACs:totalanthocyanins (TAcy) 5.65 13.7 PACs:Quercetin 192.6 161 PACs:Quercetingalactoside 27.0 18.8 (QuercGalac) PACs:Quercitrin 110.4 91.4PACs:Myricetin 147.5 135.2 PACs:Quercetin arabanoside 83.2 72.7(QuercAraban)

As shown in Table 5, the levels of total PACs and total phenolics areincreased in extract as compared to CCE feedstock. This observationconfirms that the processes disclosed herein can be used to concentratephenolics. Table 5 also presents data confirming that the levels ofsugars and organic acids are reduced in extract as compared to CCEfeedstock. This observation validates the data shown in Table 3, thatextract contains reduced amounts of sugars and organic acids, andconfirms that the processes disclosed herein can be used to separatephenolic compounds from sugars and acids and obtain a phenolics extract.

As shown in Tables 5 and 6, the ratio of PACs to total phenolics presentin feedstock is preserved in extract. Similarly, the ratio of PACs toquercetin, quercgalac, quercitrin, myricetin, and quercaraban present infeedstock are also preserved in extract. In contrast, althoughanthocyanins are present in extract, the ratio of PACs to anthocyaninspresent in feedstock is not preserved in extract (see PACs:TAcy).

Therefore, the data presented herein demonstrate that the processesdisclosed herein can be used to obtain an extract containinganthocyanins and PACs, wherein PACs oligomers are present at levelspresent in the feedstock, and wherein the ratio of PACs to totalphenolics and PACs to PACs to quercetin, quercgalac, quercitrin,myricetin, and quercaraban in extract are the same as the ratios for thesame phenolics in feedstock.

Example 2 Process Optimization

Certain steps or materials used in the process described in Example 1were substituted in an attempt to further optimize the process. Briefly,the process described in Example 1 was repeated with the followingmodifications: (1) the wash step was performed using water instead ofusing 5% ethanol; (2) the wash step and elution step were performedusing acetone; (3) the Amberlite™ XAD-7HP resin was substituted forFPX-66 resin and the wash step was performed using water instead ofusing 5% ethanol; (5) the Amberlite™ XAD-7HP resin was substituted forFPX-66 resin; and (6) the Amberlite™ XAD-7HP resin was substituted forFPX-66 resin and the wash step and elution step were performed usingacetone. The yield of phenolics extracted, extract purity, and extractstability were then assessed. The results are shown in Table 7.

TABLE 7 Process Optimization Modification # (see text in Ex. 3) 1 2 3 45 6 Resin XAD- XAD- XAD- FPX-66 FPX-66 FPX-66 7HP 7HP 7HP Wash Water 5%EtOH 5% Water 5% EtOH 5% acetone acetone Elution 90% 90% 90% 90% 90% 90%EtOH EtOH acetone EtOH EtOH acetone Yield Data PAC Yield (% 92.59 97.57101.44 86.32 88.43 96.76 of feed-Av) Phenolic 77.98 77.78 79.35 67.4580.37 84.55 Yield (folin- Av) Purity Data PACs (% dwb- 51.49 64.40 71.0241.66 42.96 44.76 Av) Phenolics 48.68 51.34 51.88 36.55 38.83 41.32(Folin-Av) Phenolics 47.55 61.86 67.70 32.74 38.43 39.72 (Folin-Bruns)Phenolics 93.97 155.63 108.32 81.56 88.12 87.45 (HPLC-OS) Acys (ppm-65056 83085 46977 58855 66269 50301 OS) Quercetin 4120 Not done Not done3180 3300 3140 (ppm-OS) ORAC 8131 9701 10379 6756 7410 6633 (Bruns)Beverage Stability Bev Haze 2.87 1.52 1.93 2.44 2.14 2.68 Slope v. timeBev Color −3.27 −4.66 −3.35 −3.50 −1.92 −2.65 Slope v. time

As shown in Table 6, both XAD-7HP and FPX-66 resin yielded high levelsof PACs and total phenolics. Overall, however, XAD-7HP provided higherresults than FPX-66, including haze stability. In contrast, FPX-66provided slightly better results for color stability. Additionally, allwash and elution conditions tested provided good results. Acetone wasobserved to be the best wash and elution solution for PAC and phenolicsrecovery and purity.

These results suggest that while the process described in Example 1 iseffective for obtaining extract containing anthocyanins and PACs,wherein PACs oligomers are present at levels present in the feedstock,and wherein the ratio of PACs to total phenolics and PACs to PACs toquercetin, quercgalac, quercitrin, myricetin, and quercaraban in extractare the same as the ratios for the same phenolics in feedstock; theprocess may be modified using the changes shown here withoutcompromising efficiency.

Example 3 Comparison of Small Scale and Large Scale Extractions

Experiments were performed to confirm that the process described inExample 1 can be performed on a large scale (i.e., a commercial scale)and that extracts obtained using large scale process represent thoseextracts described in Example 1.

The volumes of materials used in a single cycle of a large scale processare shown in Table 7.

TABLE 7 Volumes Used in Single Cycle of Small Scale Process Large(Commercial) Scale (gallons unless shown) Column Volume 141 Volume ofResin in Column 133 Volume of feedstock (50 Brix) 346 Volume ofFeedstock (25 Brix) 692 Volume of feedstock not collected 100 asfeedstock flowthrough Volume of reduced- 592 phenolics feedstock flowthrough Volume of Wash Solution 282 Volume of Elution Solution 346 Flowrate (all steps) 2.2 gallons/minute Re-equilibration volume 378

Extracts obtained using a small scale process were then compared toextract obtained using large scale process.

Briefly, a small scale process was performed using cranberry CCE asdescribed in Example 1 using the volumes shown in Table 2 and a largescale process was performed using the process described in Example 1with the volumes shown in Table 7.

A schematic representation of the above process is provided in FIG. 1.FIG. 1 includes points at which certain samples were taken. The requiredcharacteristics of each of the samples shown in FIG. 1 are detailed inTable 9.

Extracts resulting from small scale and large scale extractions werethen analyzed and compared. The results of these studies are shown inTable 8.

TABLE 8 Comparison of Extracts Obtained Using Large Scale and SmallScale Extraction Sample ID Large Small Small Small Small Small SmallSmall Small Scale Scale 1 Scale 2 Scale 3 Scale 4 Scale 5 Scale 6 Scale7 Scale 8 Solids (%) 97.25 96.15 97.44 97.2 96.45 95.72 96.02 97.1397.85 Total organic acids 0.85 0.42 0.80 0.61 0.62 0.26 0.31 0.27 0.31(%) Total Sugars (%) 0.75 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 PACs(DMAC) 61.61 91.26 67.98 82.89 80.44 97.86 77.60 81.13 85.17Anthocyanins (HPLC) 10.96 6.59 7.12 7.94 7.18 6.71 6.66 5.99 5.12 TotalPhenolics (Folin) 51.39 54.79 47.58 51.98 57.54 58.73 55.02 5.99 5.12

As shown in Table 8, the results reported in Example 2 and Table 4,obtained using small scale processes were reproducible in multiple smallscale processes and an commercial scale extraction.

These results suggest that extract containing anthocyanins and PACs,wherein PACs oligomers are present at levels present in the feedstock,and wherein the ratio of PACs to total phenolics and PACs to PACs toquercetin, quercgalac, quercitrin, myricetin, and quercaraban in extractare substantially the same as the ratios for the same phenolics infeedstock can be obtained using large scale processes.

TABLE 9 Sample Characteristics of Extraction Process Sample IDDescription Analysis Specification A CCE 1 Haze Total plate <1000/g count (TPC) Yeast <100/g Mould <100/g B Reverse Osmosis TPC <1000/g water Yeast <100/g mould <100/g C CCE 2 % Solids Read and record (RR)(%)°Brix RR (%) Specific gravity RR (g/mL) D Liquid feedstock % Solids 25 ±1% for feeding to PACs ≧1.5% dwb resin column Phenolics RR (% dwb) %titratable RR (%) acidity Haze RR (NTU) Anthocyanins RR (ppm) TPC<1000/g  Yeast <100/g Mould <100/g E Wash solution % Etoh 5 ± 1% (v/v) FPhenolics free °Brix ≧2 permeate G Elution solution % water ≦20&% byKarl Fisher H Feedstock flow % solids RR (%) through PACs RR (% dwb) TPC<1000/g  Yeast <100/g Mould <100/g I Evaporated % Solids 25 ± 2% extract1 % Etoh RR (%) J Evaporated % Solids 25 ± 2% extract 2 % Etoh RR (%) KLiquid extract % Solids 25 ± 2% Etoh <90 ppm PACs  ≧56% dwb Phenolics R(% dwb) Anthocyanins RR (% dwb) Appearance Deep red/purple liquid AromaCranberry aroma TPC <1000/g  Yeast <100/g Mould <100/g

Example 4 Use of Extracts in Food Preservation

Experiments were performed to determine whether the combination ofextract obtained using the processes disclosed herein and fumaric acidcan be used to reduce spoilage.

Briefly, various juices were inoculated with approximately 100 spores ofa mixture of the ACB strains listed in Table 10. Spores were thenharvested from PDA (Potato Dextrose Agar) plates and heat inactivated.

TABLE 10 ACB Strains Used ID Source (origin) 230 Hassia apple juice 231NFPA (National Food Processors Association) 233 Hassia apple juice 245Craving-less sugar Tropical 247 Peach juice 250 Pink grapefruit 100%juice

Extract suitable for use in the methods disclosed in this Example can beobtained using any convenient technique and from any suitable feedstock.In this case, extract was obtained from cranberries, which are aparticularly rich source of PACs, using the processes disclosed herein.Briefly, cranberry juice at 25 Brix was loaded on a Rohm & Haas XAD-7HPresin column. The column was washed with a solution containing 5%ethanol/95% water to elute sugars, acids and other unwanted components.Extract was eluted by washing the column using a solution containing 90%ethanol and 10% water. Eluate was concentrated by evaporation to 25%solids, then spray dried into a powder. The PAC level in the powderranged from 55 to 85% by weight.

PAC concentration was assessed for the experiments in this Example asfollows. Briefly, a sample to be analyzed was applied to a SephadexLH-20 column. The column was then washed with distilled water and then25% ethanol/75% water. These washes elute sugars, organic acids,anthocyanins and monomeric phenolic compounds. The PACs were then elutedby washing the column with 70% acetone in 30% water. The eluate isallowed to react with a solution of 0.1% dimethylaminocinnamaldehyde(DMAC) in 30% hydrochlonic acid in 70% methanol. DMAC can act as anelectrophile condensing with aromatic rings in an acidic media and ishighly specific for flavanols. When a prepared DMAC reagent is added toa solution containing PACs an aldehyde condensation reaction occurs withthe terminal monomer of a polymeric proanthocyanidins at the eightcarbon position of the flavanoid A-ring. The resulting colored adductshave a maximum absorption of 640 nm. A standard curve of absorbance at640 nm was developed using solutions of known contents of purifiedcranberry PACs.

Sample juices were supplemented with either cranberry juice, extract,fumaric acid, or both, in the presence and absence of about 100 ACBspores/ml, as shown in Table 11. Sample juices were then stored at 43°C. Aliquots of juice were removed periodically and tested for ACB bysubculturing onto PDA plates. Sensory testing was also performed by apanel of 2-4 people trained to detect the presence of guaiacol. Thetests were performed at several intervals during a 1 month period.Negative controls (non-inoculated juice) were included in the tests andtested at the same time as the inoculated product.

TABLE 11 Food Preservation Results % Extract Fumaric % Mg acid CranberryTA Micro Sample juice PAC/8 oz (wt/wt) juice pH Brix (%) (Growth)Sensory CranPomBlue 0 0 7.33 3.56 12.52 0.45 + + CranPomBlue 40 0 7.333.5 10.54 0.48 − − Tropical 0 0.14 0 3.43 13.35 0.46 + + Citrus Tropical40 0.14 0 3.4 13.4 0.46 − − Citrus Tropical 30 0.14 0 3.41 13.13 0.46 −− Citrus Tropical 20 0.14 0 3.41 13.18 0.46 − − Citrus Tropical 10 0.140 3.42 13.21 0.46 − − Citrus Extract water 40 0 0 2.67 2.74 0.17 − −beverage Tropical with 5 0.14 + Ruby Red Tropical with 7.5 0.14 − RubyRed Tropical with 9.5 0.14 − Ruby Red Tropical with 10 0.14 − Ruby RedTropical with 10.5 0.14 − Ruby Red Microbiological results [+] =microbial growth; [−] no growth detected. Sensory results [+] =off-flavors, typical of the presence of guaiacol; [−] no off-flavors.

As shown in Table 11, the combination of extract and fumaric acid tojuices prevented ACB growth and guaiacol production. Similar resultswere observed with apple juice and orange juice.

These observations support that the combination of extract and fumaricacid results in a synergistic effect at some concentrations.Specifically, the reduction in contamination promoted by the combinationof extract and fumaric acid is greater than the additive effect of thecomponents. This synergism was further evaluated in the followingexample.

Example 5 Minimum Inhibitory Concentration of Compositions ComprisingExtract and Fumaric Acid

The ability of the compositions described in Example 4 (i.e.,compositions comprising extract and fumaric acid) to inhibit ACB growthwere evaluated over a range of different concentrations to establish theminimum inhibitory concentration (MIC) of the composition.

Extract was obtained using the processes described herein and contained55% PACs as determined using DMAC. Fumaric acid was obtained from acommercial vendor (Tate and Lyle, IL, lot number FT7C2301B4).

Apple juice was inoculated with spores from a mixture of ACB strains(eight strains of A. acidoterrestris and one ACB strain obtained fromapple juice). These spores were suspended in phosphate buffered saline(PBS) and heat shocked at 76° C. for 10 minutes. Spore concentration wasdetermined using a hemocytometer under phase contrast microscopy. Juiceswere inoculated with 1000 spores/mL. Inoculated samples were incubatedfor 48 hours and were then cultured on acidified potato dextrose agar(PDA+TA) at 43° C. for 72 hours. Colony forming units were then counted.The ratios of extract to fumaric acid used in these experiments areshown in Table 12.

TABLE 12 Ratio of Extract to Fumaric Acid Extract (mg PAC/8 oz) Fumaricacid (%) 0 1.0 2.0 3.0 4.0 0 0/0   1/0   2/0   3/0   4/0   0.05 0/0.051/0.05 2/0.05 3/0.05 4/0.05 0.10 0/0.10 1/01.0 2/0.10 3/0.10 4/0.10 0.150/0.15 1/01.5 2/0.15 3/0.15 4/0.15 0.20 0/0.20 1/0.20 2/0.20 3/0.204/0.20

Data was plotted in the format of a isobologram. Such graphs are usefulin assessing synergy. Specifically, two compounds that result in anadditive effect yield a straight line. Deviation to the left of thisline indicates that the combination of two compounds is synergistic,while deviation to the right of the line indicates that the combinationof the two compounds is antagonistic (Vigil et. al., Methods foractivity assay and evaluation of results, Antimicrobials in Food, 3^(rd)Edition, CRC Press, Edited by Davidson, Sofos, and Branen, 2005).

As shown in FIG. 2, compositions comprising extract and fumaric acidinhibited ACB growth at a range of concentrations. Specifically, ACBgrowth was inhibited at concentrations of 0.1% fumaric acid and 0.01 mgPAC/8 oz. Furthermore, FIG. 2 shows a clear synergistic effect atconcentrations of between 0.15%-0.1% fumaric acid and 0.01-1.99 mg PAC/8oz. As noted above, the results shown in FIG. 2 represent experimentsperformed using apple juice.

Example 6 Log Reduction Experiments

Inhibition of ACB growth by compositions comprising extract and fumaricacid was confnined in 100% apple juice (Ocean Spray—see FIG. 3) and 100%orange juice (Ocean Spray—see FIG. 4). Compositions tested for eachjuice type are shown in Table 13.

TABLE 13 Ratio of Extract to Fumaric Acid Extract (mg PAC/8 oz) Applejuice Orange juice Fumaric acid (%) 0.0 2.5 0.0 12.0 0.0 0.0/0.0 2.5/0.0  0.0/0.0  12.0/0.0 0.07 — — 0.0/0.07   12/0.07 0.14 0.0/0.142.5/0.14 — —

Apple juice was inoculated with nine strains of ACB (eight strains of A.acidoterrestris and one ACB strain obtained from apple juice) and orangejuice was inoculated with four strains of A. acidoterrestris. Sporeswere heat shocked and enumerated as described in Example 5. Juicesamples were then inoculated with 500 spores and incubated at 43° C. for48 hours (apple) or 72 hours (orange) before being plated on PDA+TA.Plates were incubated at 43° C. and CFU for 72 hours. CFU werequantified as described in Example 5. CFU counts were log transformedand analyzed by to analysis of variance (ANOVA) followed by Fisher's LSDmultiple comparison test. Log reductions were calculated for fumaricacid and extract alone and in combination. Results are shown in FIGS. 3and 4.

As shown in FIGS. 3 and 4, the magnitude of ACB inhibition by extractand fumaric acid was quantified in apple juice and orange juice. Bothextract and fumaric acid significantly inhibited ACB growth in applejuice (FIG. 3A). The combination of extract and fumaric acid, howeverresulted in a synergistic reduction in ACB growth (ANOVA: p<0.001).There was a six log reduction in ACB growth in apple juice with thecombined application of extract and fumaric acid (FIG. 3B). These datasupport that the combination of extract and fumaric acid synergisticallyreduced the growth of ACB in apple juice, resulting in a >6 logreduction in the number of CFU.

As shown in FIG. 4, in orange juice, ACB was inhibited by fumaric acidalone. Furthermore, the combination of extract and fumaric acidsynergistically inhibited ACB in orange juice (ANOVA: p<0.001). Therewas no effect of extract alone (FIG. 4A). The combination of extract andfumaric acid resulted in >2 log reduction in ACB growth (FIG. 4B). Thesedata support that the combination of extract and fumaric acidsynergistically reduced the growth of ACB in orange juice, resulting ina >2 log reduction in the number of CFU.

Accordingly, the data presented herein support that the combination ofextract and fumaric acid can used effectively to reduce spoilage offruit juices by ACB.

Other Embodiments

It is to be understood that while the invention has been described inconjunction with the detailed description thereof, the foregoingdescription is intended to illustrate and not limit the scope of theinvention, which is defined by the scope of the appended claims. Otheraspects, advantages, and modifications are within the scope of thefollowing claims.

1. A method of extracting phenolics comprising: (i) obtaining a liquidfeedstock comprising cranberry juice obtained by counter currentextraction; (ii) contacting the feedstock with a resin that bindsphenolics, and that does not substantially bind to sugar and organicacids, for a time and under conditions sufficient for phenolics in thefeedstock to bind to the resin, wherein the resin has a surface area ofgreater than or equal to about 300 m²/g; (iii) contacting the resin witha wash solution, wherein the wash solution does not substantially reducethe amount of phenolics bound to the resin; (iv) contacting the resinwith an elution solution comprising a solvent, wherein the elutionsolution substantially decreases the amount of phenolics bound to theresin; and (v) collecting the elution solution.
 2. The method of claim1, wherein the elution solution comprises phenolics at a firstconcentration and further comprising: (vi) removing solvent from theelution solution; or (vii) concentrating the solution, thereby providinga liquid extract comprising phenolics at a second concentration, whereinthe second concentration is greater than the first concentration, andwherein the solution comprises at least anthocyanins andproanthocyanidins (PACs) and one or more of the following: a ratio ofanthocyanins to PACs of about 1:5; a PACs oligomeric profile that issubstantially the same as the PACs oligomeric profile in cranberries; aratio of PACs to total phenolics that is substantially the same as theratio of PACs to total phenolics in cranberries; a ratio of PACs toanthocyanins that is not the same as the ratio of PACs to anthocyaninsin cranberries; phenolics with an average molecular weight of less than14,000 Daltons; less than about 5% organic acids; and/or less than about5% sugars.
 3. The method of claim 1, further comprising drying thesolution, to thereby provide a dry extract.
 4. The method of claim 1,wherein the resin is an aliphatic ester resin and/or has moistureholding capacity of about 61% to about 69%. 5-7. (canceled)
 8. Themethod of claim 1, wherein the resin has a surface area of greater thanor equal to about 700 m²/g.
 9. The method of claim 1, wherein the resinis a macroreticular aromatic polymer.
 10. (canceled)
 11. The method ofclaim 1, wherein the wash solution comprises water or mixture of waterand ethanol at a concentration of about 5% by volume.
 12. The method ofclaim 1, wherein the elution solution comprises about 95% ethanol byvolume or about 90% acetone by volume.
 13. The method of claim 1,further comprising adding the elution solution, liquid extract, or dryextract to a liquid suitable for ingestion. 14-15. (canceled)
 16. Acomposition comprising the elution solution obtained using the method ofclaim
 1. 17. A composition comprising the liquid extract obtained usingthe method of claim
 2. 18. A composition comprising the dry extractobtained using the method of claim
 3. 19. An extract comprising at leastanthocyanins and proanthocyanidins (PACs) and at least one of thefollowing: a ratio of anthocyanins to PACs of about 1:5; a PACsoligomeric profile that is substantially the same as the PACs oligomericprofile in cranberry juice or cranberries; a ratio of PACs to totalphenolics that is substantially the same as the ratio of PACs to totalphenolics in cranberry juice or cranberries; a ratio of PACs toanthocyanins that is not the same as the ratio of PACs to anthocyaninsin cranberry juice or cranberries; phenolics with an average molecularweight of less than 14,000 Daltons; less than about 5% organic acids;and/or less than about 5% sugars.
 20. The extract of claim 19, whereinthe extract is a liquid extract.
 21. The extract of claim 19, whereinthe extract is a dry, dehydrated, or powdered extract.
 22. (canceled)23. A composition comprising isolated fumaric acid and isolatedphenolics, wherein the isolated phenolics comprises proanthocyanidins(PACs) and wherein the ratio of fumaric acid to PACs is between about4000:1 to about 100:1.
 24. The composition of claim 23, wherein theratio is about 135:1.
 25. The composition of claim 23, wherein the ratiois about 238:1.
 26. (canceled)
 27. A composition comprising isolatedfumaric acid and isolated phenolics, wherein the isolated phenolicscomprises proanthocyanidins (PACs) and wherein the ratio of fumaric acidto PACs is between about 10:1-50:1.
 28. The composition of claim 27,wherein the ratio is about 14:1. 29-62. (canceled)